Determination of the content and the molecular weight of Mussel Polysaccharide by GPC / 中国生化药物杂志
Chinese Journal of Biochemical Pharmaceutics
; (6): 9-12, 2016.
Article
in Zh
| WPRIM
| ID: wpr-506609
Responsible library:
WPRO
ABSTRACT
Objective To develop a gel permeation chromatography method for determination of content and molecular weight ( Mw ) of Mussel Polysaccharide.Methods Using GPC method, the sample was separated with TSK-gel GMPWXL(7.8 mm ×300 mm) chromatography column which was set at 35℃.The mobile phase was 0.05 mol/mL NaNO3(including 0.05%Na2N3) and the flow rate was 0.6 mL/min.The detector was RID-20AT. Results The average molecular weight of the polysaccharide of Mytilus coruscus was 1 261 411 and the average content was 88.6%by using of the calibration curves of dextrans.The average molecular weight of the polysaccharide of Mytilus edulis was 1 244 062 and the average content was 87.4%. Conclusion The method established in this paper is simple and rapid, accurate and reproducible, which can be used for the quality control of Mussel Polysaccharide.
Full text:
1
Index:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Biochemical Pharmaceutics
Year:
2016
Type:
Article