IL-4 Activated Macrophages Promote Hela Cell Migration and Invasion by Producing CCL18 / 中山大学学报(医学科学版)

ABSTRACT

Objective]To investigate the roles of IL-4 activated macrophages on Hela cells migration and invasion and the underlying mechanism.[Methods]The phenotype of IL-4 activated macrophage was assayed by morphology ,flow cytometry and Elisa. The ability of Hela cell migration and invasion was evaluated by transwell assay. The signaling proteins expression was displayed by Western blot.[Results]Il-4 activated macrophage had the phenotype of CD206high/HLA-DRlow ,and highly expressed IL-10 and CCL18 ,and decreased the expression of IL-12. The activated macrophages significantly promoted the migration and invasion of Hela cells ,while the unactivated macrophages did not had this effect. Applying anti-CCL18 antibody but not isotype IgG in the supernatant of IL-4 activated macrophage inhibited Hela cells motility. Furthermore,activated macrophages phosphorylated IKK and IκB and induced the translocation of p65 to nucleus ,eventually increased the expression of vimentin. Treatment of Hela cells with CCL18 also enhanced tumor cells migration and invasion ,as well as the NF-κB activation and vimentin expression.[Conclusion]IL-4 activated macrophages promoted Hela cells motility by secreting CCL18 and the following activation of NF-κB signaling proteins.