Effect of low expression of miR-21 on apoptosis of HepG2 cells induced by matrine / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12): 284-288, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-507431
ABSTRACT
AIM:
To explore whether miR-21 low expression enhances the effect of matrine ( MAT) on the ap-optosis of hepatocellular carcinoma cells .METHODS:
Real-time fluorescence quantitative PCR ( RT-qPCR) was used to detect the expression of miR-21 in the HepG2 cells treated with different concentrations of MAT .The effect of miR-21 on MAT-induced HepG2 cell apoptosis was analyzed by flow cytometry .The mRNA and protein expression of Bcl-2 and Bax in the HepG2 cells treated with MAT was determined by RT-qPCR and Western blot .RESULTS:
The expression of miR-21 increased with the increasing concentration of MAT .Low expression of miR-21 promoted MAT-induced apoptosis , and en-hanced the expression of Bax at mRNA and protein levels ( P<0.05 ) , while inhibited the expression of Bcl-2 at mRNA and protein levels (P<0.05).CONCLUSION:
Low expression of miR-21 enhances MAT-induced HepG2 cell apoptosis by inhibiting the expression of Bcl-2 and promoting Bax expression .
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Pathophysiology
Year:
2017
Type:
Article
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