Determination of Related Substances in Piperazine Ferulate by HPLC / 中国药师
China Pharmacist
;
(12): 351-354, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-507557
ABSTRACT
Objective:
To establish an HPLC method for the determination of related substances in piperazine ferulate.Methods:
An HPLC method was used to determine the related substances in piperazine ferulate. The separation was performed on an Xtimate C18 column (250 mm × 4. 6 mm, 5 μm). The mobile phase was 0. 5% acetic acid-methanol-acetonitrile with gradient elution. The flow rate was 1. 0 ml·min-1 and the column temperature was 30℃. The detection wavelength was 286 nm and the injection volume was 20μl.Results:
Ferulic acid had a good linear relationship within the range of 5-30 μg·ml-1(r=1.0000). The detection limit was 0. 02 ng.Conclusion:
The method is reliable, simple, accurate, stable and durable, and suitable for the determination of related sub-stances in piperazine ferulate.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacist
Year:
2017
Type:
Article
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