Analysis of Diosgenin Content in Different Processed Products of Polygonatum from Shaanxi / 中国药师
China Pharmacist
;
(12): 158-160, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-507989
ABSTRACT
Objective:
To study the effects of different processes on diosgenin content in polygonatum from Shaanxi.Methods:
The content of diosgenin was determined by HPLC on a column of Woburn C18 (250 mm × 4. 6 mm, 5 μm) with the mobile phase of acetonitrile-water(90 ∶10)at the flow rate of 1.0 ml·min-1, the detection wavelength was 203 nm, the column temperature was 30℃, and the injection volume was 30 μl.Results:
The linear range of diosgenin was 0. 892-5. 352 μg(r=0. 999 9), and the aver-age recovery was 99. 5% (RSD=2. 53%). The content of diosgenin was significantly various among the samples of polygonatum with different processes from Shaanxi (P<0. 05), and that in the raw slices was the highest followed by the steam samples, while that in al-cohol evaporate slices was the lowest.Conclusion:
The results of the research can provide reference for the quality control and process-ing methods of polygonatum from Shaanxi.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacist
Year:
2017
Type:
Article
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