Preparation of VEGFRⅡ-mediated Targeted Microbubble Image Agent and its Cancer Targeting Ability in vitro / 中国药师
China Pharmacist
;
(12): 73-76, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-508117
ABSTRACT
Objective:
To prepare vascular endothelial growth factor receptorⅡ ( VEGFRⅡ)-mediated targeted sulphur hexafluo-ride microbubble image agent and evaluate its targeting ability in human breast cancer MCF-7 in vitro.Methods:
The sulphur hexafluo-ride microbubble image agent was prepared by a membrane dispersion-ultrasonic method and optimized by Box-Behnken design using design-expert 8. 0. 6 software. VEGFRⅡ-mediated targeted microbubble image agent was prepared by an absorption method. VEGFRⅡ-mediated targeted microbubble image agent was characterized by the morphology, particle size distribution and zeta potential. The targeting ability of the image agent in human breast cancer MCF-7 in vitro was detected by an immunofluorescent assay.Results:
The particle size, polydispersion index and zeta potential of VEGFRⅡ-mediated targeted microbubble image agent was (3. 81 ± 0. 32) μm, 0. 261 ± 0. 037 and ( -25. 7 ± 2. 8) mV, respectively. The microbubbles were small and spherical with smooth surface as seen under a transmission electron microscope. VEGFRⅡ-mediated targeted microbubble image agent could combine with human breast cancer MCF-7 specifically in vitro.Conclusion:
The absorption preparation technology of the targeted microbubble image agent is feasible, and the microbubble image agent has strong and especial targeting function in vitro.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacist
Year:
2017
Type:
Article
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