Effect of miR-211 on proliferation and cell cycle related proteins of epithelial ovarian cancer cell line HO8910 / 中国免疫学杂志

ABSTRACT

Objective:To investigate the relationship between miR-211 and the occurrence of epithelial ovarian cancer,and its influence on the proliferation of ovarian cancer cells. Methods:To analyze the expression miR-211,CDK6 and Cyclin D1 of 30 cases of ovarian cancer and ovarian cancer cell lines,and 30 cases of non-ovarian cancer tissues and the normal ovarian epithelial cells were selected as the control group,and to analyze effects of miR-211 on the proliferation of ovarian cancer cells,as well as the Cyclin D1 and CDK6. Results:miR-211 relative expression level of ovarian cancer tissue was significantly lower than that in the normal group ( P<0. 05). Relative expression level of miR-211 of ovarian cancer cells was significantly lower than that in normal ovarian epithelial cells (P< 0. 05);in epithelial ovarian cancer cell line HO8910,cell number of miR-211 on the 3-day and the 4-day was significantly lower than that of miR-Ctrl group (P<0. 05);relative expression levels of Cyclin D1 and CDK6 in epithelial ovarian cancer were significantly higher than those in normal ovarian epithelial tissues (P<0. 05);miR-211 of epithelial ovarian cancer cell lines significantly inhibited Cyclin D1 and CDK6 expression;in ovarian cancer tissues,Spearman correlation analysis results showed that relative expression levels of miR-211 and Cyclin D1 and CDK6 was negatively correlated ( r=-0. 583, P= 0. 010 ) . Conclusion: miR-211 can inhibit the proliferation of ovarian cancer cells,and inhibit the expression of Cyclin D1 and CDK6;miR-211,Cyclin D1 and CDK6 in ovarian cancer may be involved in the regulation of ovarian cancer.