Effects of miR-581 overexpression on proliferation of human colorectal cancer SW620 cells / 中国免疫学杂志
Chinese Journal of Immunology
;
(12): 252-255, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-508282
ABSTRACT
Objective:
To investigate the role of miR-581 overexpression on the proliferation of human colorectal cancer cell line SW620.Methods:
The expression group,colorectal cancer SW620 cells were transfected with recombinant lentivirus vector ( LV-miR-581) and miR-581 mimics(miR-581),the negative control group were transfected with negative control lentiviral vector (LV-GFP) and negative control mimics (vector). The mRNA expression of miR-581 was identified by qRT-PCR. Proliferation of the cells were detected by CCK8 assary and colony forming assary.Results:
The expression of miR-581 at mRNA significantly increased in LV-miR-581 group compared with control groups were detected by qRT-PCR ( P<0. 05 ) . Up-regulation of miR-581 markedly enhanced human colorectal cancer SW620 cells proliferation than those in the cells transfected with control vector ( P<0. 05 ) .Conclusion:
Forced expression of miR-581 accelerates the proliferation of colorectal cancer SW620 cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
2017
Type:
Article
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