Effect of sevoflurane on learning and memory function and apoptosis of hippocampus in infant rats / 中国免疫学杂志

ABSTRACT

Objective:To explore the effect of sevoflurane on learning and memory function and apoptosis of hippocampus in infant rats. Methods:100 SD rats aged 6-8 days were randomly divided into 5 groups(n=20),the group Ⅰinhaled 3% sevoflurane and 25% oxygen for 2 hours;the groupⅡinhaled only 25% oxygen for 2 hours;the groupⅢinhaled 3% sevoflurane and 25% oxygen for 2 hours on the 6th,7th,8th day after birth;the group Ⅳ inhaled 25% oxygen for 2 hours on the 6th,7th,8th day after birth;groupⅤ were just put into the anesthetized box without any treatment. When the rats were grown up to 16-21 days after anesthesia,the effect of learning and memory function were assessed by Morris water maze test,and activity of caspase-3 were detected by Western blot,the apoptosis of hippocampus were detected by tunnel. Results:①Compared to groupⅣ,the mean escape lantency of groupⅠshowed no significant changes(P>0. 05),while group Ⅲ was significant longer (P<0. 05),which was significant different from other groups(P<0. 05).②In the space exploration experiment,there was no difference between group Ⅰ and Ⅳ,but compared to group Ⅰ and other groups,the number of cross platform of groupⅢwas decreased (P<0. 05).③The swimming speed of groupⅢwas significantly slower than the other groups.④Compared to group Ⅳ,the activity of caspase-3 slightly increased in group Ⅰ,and significantly increased in group Ⅲ( P<0. 05 ) . ⑤The positive cells in hippocampus stained by TUNEL in group Ⅲ was significant increased ( P<0. 05 ) . Conclusion:Learning and memory function of infant rats aged 6-8 days are impaired after three exposures to sevoflurane when they grow up to 16-21 days,and this may relate to the apoptosis of hippocampus.