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Mechanism of thiocoraline combination with cisplatin on promoting apoptosis of cervical cancer cell line c4-1 and Hela / 中国免疫学杂志
Chinese Journal of Immunology ; (12): 99-102, 2017.
Article in Chinese | WPRIM | ID: wpr-508444
ABSTRACT

Objective:

To investigate the apoptosis by thiocoraline in combination with cisplatin on cervical cell line c4-1 and Hela and its mechanism.

Methods:

Different concentrations of thiocoraline and cisplatin alone or in combination used for cultured c4-1 and Hela cells. The inhibition of cell proliferation was detected by MTT assay,and the apoptosis was detected by Hoechst 33342 and AO-EB staining. The expressions of the protein Bax,Bcl2,p53,p21 were detected by Western blot. The cell cycle of c4-1 and Hela cells were detected by Flow cytometry.

Results:

The cell proliferation activity of c4-1 and HeLa cells were inhibited obviously by different concentrations of thiocoraline and cisplatin alone or in combination. The inhibition effect was in a dose-dependent manner. Meanwhile, when the concentrations of thiocoraline and cisplatin were 10 nmol/L and 4 μmol/L, respectivity the inhibitory effect was most significant (P<0. 05);Hoechst 33342 and AO-EB staining showed that the cell apoptosis effect was more obvious on thiocoraline in combination with cisplatin. The Western blot showed that the expressions of the protein Bax,p53,p21 were significantly up-regulated, and the Bcl2 was significantly down-regulated.

Conclusion:

Thiocoraline combination with cisplatin could up-regulate the expression of the protein Bax,p53,p21 and down-regulation the expression of Bcl2 to inhibition the proliferation and promotion apoptosis of cervical cell line c4-1 and Hela.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Immunology Year: 2017 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Immunology Year: 2017 Type: Article