Biological characteristics of bone marrow mesenchymal stem cells in patients with or whithout coronary heart disease:a comparative study / 中国组织工程研究

ABSTRACT

BACKGROUND:It is unclear whether the body differences in patients with different types of heart diseases affect the characteristics and performance of stem cel s. OBJECTIVE:To explore the biological characteristics of bone marrow mesenchymal stem cel s from patients with different types of heart diseases. METHODS:Bone marrow mesenchymal stem cel s were extracted using density gradient centrifugation from the bone marrow of 27 patients with coronary heart diseases and 20 patients with other heart diseases. Cel morphology was observed in the two groups. CD13, CD34, CD45, CD54, CD106 and CD44 positive expression was detected by flow cytometry. Cel proliferation was detected by MTT method, and the in vitro cel growth curves of the two groups were described. RESULTS AND CONCLUSION:The bone marrow mesenchymal stem cel s of the two groups showed a long spindle shape, and there was no significant difference in the cel morphology between the two groups. In al the patients, the positive rates of CD34 and CD455 were less than 3.0%, while the positive rates of CD13 and CD44 were higher than 95.0%. However, the positive rates of CD54 and CD106 were higher in patients with coronary heart disease as compared with those with other heart diseases (P<0.05). The in vitro growth curves of cel s in the two groups were basical y consistent, and the cel proliferation was only a little higher in the patients with other heart diseases compared with those with coronary heart disease. Experimental results show that different types of heart diseases in patients have no influence on morphology and proliferation of bone marrow mesenchymal stem cel s, but some function-related proteins may exhibit certain difference in their expressions.