Isolation and culture of primary microvascular endothelial cells from mouse brains / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
;
(53): 7666-7671, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-508683
ABSTRACT
BACKGROUND:
Brain microvascular endothelial cel s (BMECs) are important tools in the field of neuroscience research;therefore, how to obtain highly purified BMECs is a key and difficulty in vitro.OBJECTIVE:
To develop a simple method of isolating and culturing highly purified BMECs.METHODS:
C57BL/6 mice aged 6-8 weeks old were selected, and microvessels were obtained using enzyme digestion and gradient centrifugation. Further, endothelia cel s were purified by certain drugs, fol owed by identified by CD31 and GFAP immunofluorescence staining. The expression of Claudin-5 was detected using immunofluorescence staining with anti-Claudin-5 antibody. RESULTS ANDCONCLUSION:
Mouse BMECs grew and arranged in spiral or cobblestone-like. Immunofluorescence staining showed that the purity of BMECs reached above 99%and Claudin-5 was highly expressed. In conclusion, a simple method of easy accessibility is developed to obtain highly purified primary mouse BMECs.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Tissue Engineering Research
Year:
2016
Type:
Article
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