In vitro study on a degradable poly-L-lactic acid biliary-enteric composite stent / 中国组织工程研究

ABSTRACT

BACKGROUND:The degradable poly-L-lactic acid (PLLA) biliary-enteric composite stent has been developed. OBJECTIVE:To analyze the solubility and biocompatibility of the degradable PLLA biliary-enteric composite stent. METHODS:Solubilitythe PLLA composite stent was implanted into artificial gastric acid to detect the dissolution rate within 12 weeks. Pyrogen testthe PLLA composite stent extracts were injected into the rabbits via ear vein to detect the changes of body temperature. Hemolysis testthe PLLA composite stent extracts, normal saline and distil ed water were added into the rabbit anticoagulant, respectively, to detect the hemolysis ratio. Cytocompatibility testCaco-2 cel s were respectively cultured in the DMEM medium containing 10%fetal bovine serum, rubber material extracts and the PLLA composite stent extracts, and the cel proliferation was detected at 12, 24, 36 and 48 hours;the lactic dehydrogenase release was detected at 2 days. RESULTS AND CONCLUSION:The PLLA composite stent showed a long stability in vivo, and approximately 80%was dissolved at about 20 weeks. No reactions of pyrogen and henolysis were observed in the pyrogen and hemolysis tests. The PLLA composite stent made no effects on the Caco-2 cel proliferation and lactic dehydrogenase release. In conclusion, the PLLA composite material holds a good solubility and cytocompatibility.