Evaluation of two Real-Time PCR Assays for the Detection of Chlamydia trachomatis / 임상검사와정도관리
Journal of Laboratory Medicine and Quality Assurance
;
: 43-47, 2011.
Article
in Korean
| WPRIM
| ID: wpr-50879
ABSTRACT
BACKGROUND:
Chlamydia trachomatis is common etiological agent of sexual transmitted disease. We evaluated two real-time PCR assays for the detection of C. trachomatis; Cobas TaqMan CT test v2.0 (Roche Diagnostics, Switzerland) and AdvanSure CT/NG real-time PCR (LG Life Sciences, Korea). And we compared performance of two real-time PCR assays with Cobas Amplicor CT test (Roche Diagnostics, Switzerland).METHODS:
Seventy-nine cervical swab specimens collected from patients with suspected C. trachomatis infection were tested using CTM CT, ADVANSURE, and AMPLICOR for the detection of C. trachomatis.RESULTS:
Twenty-five (31.6%) samples showed positive results in all three assays. The positive and negative concordance rates among three assays were 100%.CONCLUSIONS:
CTM CT and ADVANSURE showed excellent agreement with AMPLICOR. Therefore, these assays were reliable for the evaluation of C. trachomatis infection.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Chlamydia
/
Chlamydia trachomatis
/
Biological Science Disciplines
/
Real-Time Polymerase Chain Reaction
Type of study:
Diagnostic study
Limits:
Humans
Language:
Korean
Journal:
Journal of Laboratory Medicine and Quality Assurance
Year:
2011
Type:
Article
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