Simultaneously determining the contents of combretastatin A4 myristate and its active metabolite combretastatin A4 by HPLC / 国际药学研究杂志

ABSTRACT

Objective To develop a high performance liquid chromatography(HPLC)method for simultaneously determining the contents of combretastatin A4 myristate(CA4-14)and its active metabolite combretastatin A4(CA4)in rat plasma. Methods 1-Naphthol was used as internal standard. The analysis was performed on a Agilent Eclipse plus C18(4.6 mm × 250 mm,5μm )with gra?dient elution by using the mobile phase of acetonitrile(A)-0.4%formic acid solution(B). The column temperature was 30° C and the flow rate was 1.0 ml/min. The detection wavelength was 295 nm and the injection volume was 20μl. Results The calibration curves were linear within the range of 0.2-100μg/ml(r2=0.9980)for CA4-14,and 0.05-50μg/ml(r2=0.9999)for CA4,while the quantifica?tion limits of CA4-14 and CA4 were 0.2μg/ml and 0.01μg/ml,respectively. The recoveries of CA4-14 and CA4 were 93.79%(RSD=1.94%)and 96.81%(RSD=3.11%). Conclusion The method is convenient,fast,sensitive,with good precision,and can be used to determine the contents of CA4-14 and C A4 in rat plasma.