Determination of notoginsenoside R1,ginsenosides Rg1 and Rb1 in Xixian Tongshuan pills by HPLC / 国际药学研究杂志

ABSTRACT

Objective To establish a HPLC method for determining notoginsenoside R1,and ginsenosides Rg1 and Rb1 in Xixian Tongshuan pills. Methods C18 column was adopted with acetonitrile as mobile phase A,water as mobile phase B,gradient elution. The flow rate was 1.0 ml/min,column temperature was 35℃and detection wavelength was 203 nm. Results The linear range of notoginsenoside R1,and ginsenosides Rg1 and Rb1 was 0.412-1.855μg(r=0.9994),1.903-8.563μg(r=0.9994),and 1.822-8.201μg(r=1.0000),respectively. The total average recovery rate of notoginsenoside R1,and ginsenosides Rg1 and Rb1 was 98.48%, 97.33%and 97.29%with RSD of 1.58%,1.13%and 1.08%,respectively. Conclusion The method is reliable,accurate,and can be used for the quality control of the preparation.