Effect of lentinan polysaccharides on hippocampus in Alzheimer 's disease rats / 中国生化药物杂志

ABSTRACT

Objective To evaluate the effect of lentinan polysaccharide (LPS) on Alzheimer's disease (AD) rats, and providing a new idea for prevention and treatment of Alzheimer's disease (AD).Methods 96 SPF rats were randomly divided into control group, model group, LPS low dose-treated group, LPS middle dose-treated group, LPS high dose-treated group and positive control group(piracetam treated), each had 16 rats.The model of AD was induced by injection of Aβ25-35(80 pmol/μL), rats in four treatment groups were treated with LPS (200 mg/kg), LPS (400 mg/kg), LPS (600 mg/kg) and piracetam(600 mg/kg) orally once a day for 60 days while control group and model group were given equal-dose saline.After treatment, the ability of navigation and memory were evaluated by water maze test (MWM), and then rats in all groups were sacrificed to obtain the hippocampus.HE staining was used to observe the histopathological changes, the expressions of TACE,Aβ,BACE1 and PP2A protein in hippocampus were detected by Western blot.Results Compared with the model group, high dose LPS could significantly improve the ability of learning and memory (P<0.01) and improve the ability of locating navigation (P<0.01).HE staining results showed that LPS treatment could repair the rat hippocampus injury induced by Aβ25-35 .Western blot analysis showed that the levels of Aβ, and BACE1 were down-regulated in LPS group compared with the model group, while the protein level of PP2A and TACE was up-regulated.Conclusion LPS has a protective effect on Aβ25 -35 induced AD model, and it is expected to be a strategy for treatment of AD .