Role of NFIC on cAMP-mediated diferentiation of stem cells from the apical papilla / 安徽医科大学学报
Acta Universitatis Medicinalis Anhui
;
(6): 190-193, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-509591
ABSTRACT
Objective To investigate the role of NFIC on the stimulation effects of cAMP-induced differentiation of stem cells from the apical papilla ( SCAPs) in vitro. Methods SCAPs isolated from dental papilla of human imma-ture third molars were cultured by enzyme digestion. SCAPs were transfected with lentivirus that overexpressed NF-IC gene ( ov-NFIC) or an empty vector ( LV-empty) and co-treatment with Forskolin. Mineralized nodule formation of each group was measured by alizarin red staining. Quantitative real-time reverse-transcription polymerase chain reaction was performed to test the expressions of RUNX2,ALP,OCN mRNA. Results Forskolin increased the ex-pression of Runx2, ALP, OCN mRNA as well as matrix mineralization in SCAPs, and the stimulation effects of For-skolin were enhanced by overexpressing NFIC gene. Conclusion The results indicate that NFIC can promote cAMP-induced differentiation of SCAPs.
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Acta Universitatis Medicinalis Anhui
Year:
2017
Type:
Article
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