RIP3 expression and function in the liver of rats following ischemic postconditioning / 中国现代普通外科进展
Chinese Journal of Current Advances in General Surgery
;
(4): 5-9, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-509917
ABSTRACT
Objective:
To investigate the expression and function of RIP3 in the liver of rats following ischemic postconditioning.Methods:
The model of 70% hepatic ischemia and reperfusion was established,then a total of forty healthy adult male Sprague-Dawley(SD) rats were divided randomly into four groups,ten rats in each groupa sham-operation group (Sham group);an ischemia reperfusion injury group(IR group);an ischemic postconditioning group(IPO group);an ischemic postconditioning and necrostatin-1 group (Nec-1 group).The blood samples and liver tissues were collected.The serum levels of ALT and AST were detected,and the liver histological examination was performed.Western-bolt was used to detect the TNF-α and RIP3 levels.Results:
Compared with the IR group,ALT and AST in serum were significantly declined in the IPO group (P<0.05);The liver damage after ischemia and reperfusion was improved obviously in the IPO group compared to which in the IR group;The Suzuki's scores was increased in the IR group compared to which in the IPO group (P<0.05);There was a low grade of TNF-α and RIP3 in the Sham group,whereas the level of TNF-α and RIP3 significantly increased in the IR and IPO and Nec-1 group(P<0.05);Compared with the IR group,the level of RIP3 was further decreased in the IPO group (P<0.05);Compared with the IPO group,the level of RIP3 was further decreased in the Nec-1 group (P<0.05).Conclusion:
RIP3-mediated necroptosis was involved during hepatic ischemia postconditioning,and the protective effect of ischemia postconditioning may act as reducing necroptosis by cutting down the levels of RIP3.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Current Advances in General Surgery
Year:
2017
Type:
Article
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