Biological characterization and in vitro biocompatibility of human placenta derived mesenchymal stem cells / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
;
(53): 755-759, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-510655
ABSTRACT
BACKGROUND:
At present bone marrow mesenchymal stem cel s act as the main seed cel s in bone tissue engineering, but only 0.001%-0.01%cel s are in the bone with difficulty in cel separation and purification.OBJECTIVE:
To explore the biological characterization of human placenta derived mesenchymal stem cel s and biocompatibility with three-dimensional porous hydroxyapatite ceramic scaffold.METHODS:
Human placenta derived mesenchymal stem cel s were morphological y observed and identified usingflow cytometry, fol owed by osteogenic, adipogenic, chondrogenic induction for 3 weeks. Afterwards, the potential of multi-directional differentiation was identified by alizarin red S, oil red O and toluidine blue staining. DAPI staining was used to observe the adhesion of cel s on the surface of the hydroxyapatite ceramic scaffold under scanning electron microscope. RESULTS ANDCONCLUSION:
The human placenta derived mesenchymal stem cel s showed long spindle shape and uniform size under the microscope;they highly expressed CD29 and D90, but did not express CD45 and CD106. Fol owing induction, mineralized nodules were observed by alizarin red S staining, lipid droplets by oil red O staining and blue-dyed toluidine blue staining. These cel s adhered wel to the scaffold surface, indicting they are suitable for bone tissue engineering.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Tissue Engineering Research
Year:
2017
Type:
Article
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