Regulation of the Circadian Gene CLOCK Expression by KCl Depolarization in B35 Rat Neuroblastoma Cells / 신경정신의학
Journal of Korean Neuropsychiatric Association
;
: 21-27, 2006.
Article
in Korean
| WPRIM
| ID: wpr-51086
ABSTRACT
OBJECTIVES:
To investigate the effects of KCl on regulation of circadian gene CLOCK expression, we observed whether induction of CLOCK is influenced by KCl depolarization in B35 rat neuroblastoma cells.METHODS:
B35 rat neuroblastoma cells were grown in Dulbecco's modified Eagle's medium supplemented with 10% FBS and 1% penicillin-streptomycin in a 37 degrees C humidified incubator with 5% CO2. Inhibitors including cycloheximide and actinomycin D were pretreated 1 hour before treatment with 50mM KCl. Immunoblotting with anti-CLOCK antibody was done.RESULTS:
CLCOK is induced by 50 mM KCl in B35 Rat Neuroblastoma cells, and a maximal induction in CLOCK level reached peak at 8 to 20 hours. The pretreatment of cycloheximide and actinomycin D prevented the induction of CLOCK by 50 mM KCl.CONCLUSION:
We suggest that KCl depolarization may play critical roles in several aspects of the circadian gene CLOCK expression.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Immunoblotting
/
Cycloheximide
/
Dactinomycin
/
Circadian Clocks
/
Incubators
/
Neuroblastoma
Limits:
Animals
Language:
Korean
Journal:
Journal of Korean Neuropsychiatric Association
Year:
2006
Type:
Article
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