Intervention of Effective Parts of Acanthopanax Senticosus on the Expression of LRRK 2 in Injured PC12 Cells Induced by MPP+ / 中国药师
China Pharmacist
;
(12): 612-616, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-511703
ABSTRACT
Objective:
To observe the effects of effective parts of Acanthopanax senticosus on the expression of LRRK 2 in injured PC12 cells induced by MPP+ to explore the mechanism of neuroprotection of the effective parts of Acanthopanax senticosus.Methods:
The PC12 cell model of Parkinson's disease (PD) was constructed by MPP+,and intervened by the effective parts of Acanthopanax senticosus.The cell survival rate was detected by MTT,the mRNA expression level of LRRK 2 was studied by PCR,and the protein expression of LRRK 2 was determined by immunohistochemistry and Western Blot.Results:
Compared with that of the model group,the survival rate of the drug group increased with significant difference (P<0.01).Compared with those in the model group,the LRRK 2 gene and protein expression all decreased with significant differences (P<0.01).Conclusion:
The effective parts of Acanthopanax senticosus show protective effects on the PC12 cell model of PD,and one of the mechanisms may be related with the decreased expression level of LRRK2 caused by the effective parts of Acanthopanax senticosus.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacist
Year:
2017
Type:
Article
Similar
MEDLINE
...
LILACS
LIS