Study on the Total Flavonoids Content and Antioxidant Activity in Different Parts of Eucommiae ulmoides / 中国药房

ABSTRACT

OBJECTIVE:To compare the total flavonoids content and antioxidant activity in the barks,leaves,male flowers and seeds of Eucommiae ulmoides. METHODS:UV spectrophotometry was used to determine the total flavonoids content in differ-ent parts;tests was conducted to clear 2,2′-nitrilobis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS+),1,1-diphenyl-2-trinitro-phenylhydrazine(DPPH)radicals and the reducing ability of Cu2+,using half clear/reduction concentration value(IC50)as evalua-tion indexes,and vitamin C was regarded as positive control. RESULTS:The total flavonoids content of the E. ulmoides from hight to low was as follows as leaves>male flowers>barks>seeds,except there was no significant difference in barks and seeds (P>0.05),the other parts had significant differences (Pmale flowers>seeds>barks,except there was no significant difference in the indicators of leaves and male flowers(P>0.05),the other parts had significant differences(Pmale flowers>barks>seeds,there was significant difference in leaves and males flowers with barks and seeds (P<0.05). CONCLUSIONS:The content of total flavonoids in leaves and male flowers is high,and the antioxidant activity is strong,which has a great prospect of exploitation and utilization to make up for deficiencies in barks of E. ulmoides.