Optimization of Extraction Technology of Compound Rhodiolae crenulatae Granule by Multi-index Ortho-gonal Test / 中国药房
China Pharmacy
;
(12): 1830-1833, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-512434
ABSTRACT
OBJECTIVE:
To optimize the extraction technology of Compound Rhodiolae crenulatae granule.METHODS:
The pharmacodynamic index of learning and memory ability was used to screen the water extraction,water extraction and alcohol pre-cipitation and alcohol extraction methods for Compound R. crenulatae granule in mice by Y-type water maze test. Using the compre-hensive scores of salidroside,ferulic acid extraction amount and extraction yield as indexes,orthogonal test was used to optimize the ethanol volume fraction,extraction time,solvent amount and extraction times,and verification test was conducted.RESULTS:
Products by alcohol extraction can significantly improve learning and memory ability in the 3 methods;the optimal extraction tech-nology was confirmed to 8-fold 70% ethanol reflux extracting for 3 times,1.0 h each time. In verification test,average extraction amount of salidroside and ferulic acid were 4.884 mg/g and 1.669 mg/g,and average extraction yield was 33.52%(all RSD<2%, n=3).CONCLUSIONS:
The optimized extraction technology is stable,reliable,simple and feasible,which can be used for indus-trial extraction of Compound R. crenulatae granule.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2017
Type:
Article
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