Simultaneous determination of four constituents in Canarii Fructus by RP-HPLC / 中成药
Chinese Traditional Patent Medicine
;
(12): 773-776, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-512717
ABSTRACT
AIM To establish a RP-HPLC method for the simultaneous content determination of four constituents in Canarii Fructus.METHODS The analysis of Canarii Fructus methanol extract was performed on a 30 ℃ thermostatic Hypersil ODS column (4.6 mm × 200 mm,5 μm),with the mobile phase comprising of acetonitrile0.1% phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 237 nm.RESULTS Gallic acid,protocatechuic acid,chlorogenic acid and epicatechin displayed good linear relationships within the ranges of 0.525-10.500 μg/mL (r =0.999 9),0.700-14.000 μg/mL (r =0.999 9),0.575-11.500 μg/mL (r =0.999 9) and 0.550-11.000 μg/mL (r =0.999 9),whose average recoveries were 99.05% (RSD=1.4%),98.73% (RSD=0.8%),98.69% (RSD=1.3%) and 99.47% (RSD=1.1%),respectively.CONCLUSION This simple,sensitive and relible method can be used for the quality control of Canarii Fructus.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional Patent Medicine
Year:
2017
Type:
Article
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