miR-25 promotes gastric cancer cells proliferation by directly targeting KLF4 / 实用医学杂志
The Journal of Practical Medicine
;
(24): 867-871, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-513149
ABSTRACT
ObjectiveTo investigate whether miR-25 targets KLF4 to regulate the growth of gastric cancer cells and lucidate the mechanism of miR-25 producing functional effects in gastric cancer cells. Methods The expression levels of miR-25 and KLF4 were detected by qRT-PCR in 35 pairs of gastric cancer tissues of patients. Target genes of miR-25 were predicted using Targetscan and verified though dual luciferase activity assay and west-ern blot. MTT assay and clone formation assay were detected after downregulated miR-25 and overexpressed KLF4 respectively. Results miR-25 was highly expressed in gastric cancer tissues with KLF4 downexpresstion. Downreg-ulated miR-25 and overexpressed KLF4 respectively can significantly reduce the proliferation of gastric cancer cells,the results were opposite when overexpression of KLF4. Conclusion miR-25 promotes gastric cancer cells proliferation by targeting KLF4.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
The Journal of Practical Medicine
Year:
2017
Type:
Article
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