Ultra Performance Liquid Chromatography-Tandem Mass Spectrometric Method for Detection of Pork Peptide Biomarkers / 分析化学
Chinese Journal of Analytical Chemistry
;
(12): 205-210, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-513398
ABSTRACT
The samples of muscular tissue from pork,beef and lamb which were closely related in the genetic relationship were analyzed by ultra performance liquid chromatography-tandem mass spectrometric (UPLC-MS) technique.The specific peptide biomarkers of pig meat species were found and confirmed.Proteins from three pure meat samples were extracted and digested using trypsin,the digested proteins were identified by UPLC-triple time-of-flight (TOF)-MS,and the total ion chromatogram (TIC) was searched and analyzed against the UniProt database.Three high abundant homologous proteins of three species and 8 potential peptide biomarkers of pork were found.A multiple reaction monitoring (MRM) QTRAP-MS method was established to confirm the specificity of potential peptide biomarkers.As a result,five peptide biomarkers of pig species meat were confirmed,three of which were not reported.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
Language:
Chinese
Journal:
Chinese Journal of Analytical Chemistry
Year:
2017
Type:
Article
Similar
MEDLINE
...
LILACS
LIS