Reliability evaluation of the blast/abnormal lymphocyte alarm tested through white precursor cell (WPC) channel on the Sysmex XN-9000 analyzer / 临床检验杂志

ABSTRACT

Objective To evaluate the reliability of the white precursor cell (WPC) channel on the Sysmex XN-9000 hematology analyzer (XN-9000) in giving the abnormal white blood cell (WBC) alarm.Methods A total of 520 blood samples with abnormal WBC alarm from the Sysmex XE-2100 (XE-2100) and XN-9000 were performed the manual peripheral blood smear review,and their results were analyzed.Results The specificity of the XN-9000 WPC channel in the blast alarm was significantly higher than that of XE-2100 (x2 =6.98,P＜0.05),while the false positive rate of the XN-9000 (36％) significantly lower than that of XE-2100 (64％,x2 =6.18,P ＜ 0.05).The overall efficiency of the XN-9000 WPC channel in identifying blasts and abnormal lymphocytes (90.4％ and 94.4％) was significantly higher than that of the XE-2100 (72.2％ and 80.1％,x2 =5.6 and 6.33,P ＜ 0.05).Under the automatic reflex analysis mode of WPC channel,the specificity and overall efficiency of the WPC channel in identifying blasts and abnormal lymphoeytes of all the detected samples were significantly higher than those of the WDF channel (x2 =6.15,4.14,6.02 and 4.88,P ＜0.05).The total review rate of the XN-9000 (WDF/WPC) was significantly lower than that of the XE-2100 (x2 =4.33,P ＜ 0.05).Similarly,the review rate of the XN-9000 for the blast/abnormal lymphocyte alarm (84/260,32％) was significantly lower than that of the XE-2100 (107/260,41％) (x2 =4.38,P＜0.05).Conclusion Compared with the XE-2100,the XN-9000 WPC channel can improve the overall efficiency of the blast/abnormal lymphocyte alarm,reduce the false positive rate and the review rate,and then improve the efficiency of clinical laboratories.