Change of Expression and Activity of Heme Oxygenase-1 in Rat Corpus Cavernosum during Low-flow Priapism / 대한남성과학회지
Korean Journal of Andrology
;
: 81-86, 2004.
Article
in Korean
| WPRIM
| ID: wpr-51425
ABSTRACT
PURPOSE:
The inducible isoform of heme oxygenase(HO), HO-1, responds to hypoxia. HO-1 regulates vascular smooth muscle tone through carbon monoxide production. To investigate the possible role of HO-1 in low-flow priapism, we examined the expression and activity of HO-1 in artificially induced veno-occlusive priapism in rat. MATERIALS ANDMETHODS:
Fourteen male Sprague Dawley rats were divided into 2 groups with 7 rats each. In the first group, low-flow priapism was induced using a vacuum-constriction device and a constriction rubber band; in the second group, low-flow priapism was induced using papaverine. We measured the expression level and activity of HO-1 in penile tissues after time periods of 0(control), 2, 3, 4, 8, 12, 24, and 48 hours. At the same time, the expression levels of i-NOS, e-NOS, and beta-actin(control) in penile tissues were also measured.RESULTS:
In both groups, expression of HO-1 and HO-1 enzyme activities in penile tissue significantly increased in a time dependent fashion(p<0.01). However, there was no difference in the expression of i-NOS and e-NOS in both groups at any time period.CONCLUSIONS:
HO-1 was induced over time in rats with artificially induced veno-occlusive priapism. Induction of HO-1 may play a protective role against hypoxic injury, but may also play an important role in the vicious cycle observed for low flow priapism. Increasing induction of HO-1 against hypoxic injury in a prolonged erectile state promotes sustained dilatation of corporal smooth muscle, and this may aggravate low-flow priapism.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Papaverine
/
Priapism
/
Rubber
/
Carbon Monoxide
/
Rats, Sprague-Dawley
/
Constriction
/
Dilatation
/
Heme Oxygenase-1
/
Heme
/
Heme Oxygenase (Decyclizing)
Limits:
Animals
/
Humans
/
Male
Language:
Korean
Journal:
Korean Journal of Andrology
Year:
2004
Type:
Article
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