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Effects of Grape Seed Proanthocyanidin on the Transport of Bile Salts in Colon Glandular Cell Caco-2 / 中国药房
China Pharmacy ; (12): 1323-1325, 2017.
Article in Chinese | WPRIM | ID: wpr-515013
ABSTRACT

OBJECTIVE:

To study the effects of grape seed proanthocyanidin(GSP)on the transmembrane transport of sodium glycocholate (GA) and sodium taurocholate (TA) in colon glandular cell Caco-2.

METHODS:

Caco-2 model was used,and RP-HPLC was conducted to determine the contents of GA and TA in cell culture medium. The test was divided into GSP group, GA group,TA group,GSP+GA group and GSP+TA group,the transport volumes of transporting GA and TA from Transwell apical (AP)side to basolateral(BL)side by Caco-2 cell at 0,2,4,8 h were detected,respectively.

RESULTS:

The linear ranges of GA and TA were 0.05-1.2 mmol/L(R2=0.9999). With the time passing,transport volumes of GA and TA in BL site in GA group and TA group were sharply increased;while the transport volumes were obviously decreased after adding GSP,with statistical signifi-cance(P<0.01).

CONCLUSIONS:

GSP has inhibitory effect on the transmembrane transport of GA and TA in Caco-2 cell.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Pharmacy Year: 2017 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Pharmacy Year: 2017 Type: Article