Content Determination of Urea in Urea [13C] Capsules by High Performance Cation-exchange Chromatogra-phy / 中国药房
China Pharmacy
;
(12): 1236-1238, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-515069
ABSTRACT
OBJECTIVE:
To determine the content of urea in Urea [13C] capsules by high performance cation-exchange chroma-tography (HPCEC).METHODS:
The determination was performed on Zorbax 300 SCX column with mobile phases consisting of acetonitrile-0.1% phosphoric acid (2080,V/V) at the flow rate of 1.0 mL/min. The detection wavelength was set at 200 nm and column temperature was 35 ℃. The sample size was 20 μL.RESULTS:
The linear range of urea was 0.0039-1.0030 mg/ml(r=0.9997). The limit of quantitation was 3.918 μg/mL and the limit of detection was 0.975 μg/mL. RSDs of precision,stability and repetitive test were all lower than 2.0%. The recovery ranged 99.3%-101.0%(RSD=0.67%,n=9).CONCLUSIONS:
The meth-od is simple,rapid,sensitive and suitable for the content determination of urea in Urea [13C] capsules.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2017
Type:
Article
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