Study on unscheduled DNA synthesis of Alternaria alternata 261-B2-3 in human amnionic cells in vitro / 中国病理生理杂志

ABSTRACT

Tne Unscheduled DNA syntheris (UDS) was induced in cultured human amnionic cell line FL in vitro by exposure to 261-B2-3, an extract of Alternaria alternata separated from crops in Linxian county which is a high risk area of esophageal cancer in China. In the absence of S-9M, 261-B2-3 was capable of inducing UDS in target cells and the ratio of UDS elicited by the extract (the range of concentration being 10~(-6) to 10~(-3) mg/ml) was significatly different from the ratio by solvent control (P0.05) with S-9M. The results demonstrated that 261-B2-3 has stronger mutagenicity without S-9M and it's mutagenicity is rising with increasing concentrations in a certain range. On the contrary, 261-B2-3 has no or only a weak mutagenicity with S-9M. It is suggested that there arc direct mutagens in this extract. It is possible that Alternaria alternata contaminated the crops play an important role in the high incidence of esophageal cancer in Linxian county.