The protection of DS-182 during reperfusion of total ischernia heart / 中国病理生理杂志

ABSTRACT

The role of oxygen free radical's scavenger systems in the protection of cellular damages during total ischemia and reperfusion was studied in isolated Langendorff perfused rat hearts. The changes of myocardial enzymes(superoxide dismutase and glutathione peroxidase)and ultrastructure were used as indexes of cellular damage. The hearts were perfused in total ischemia for 40, 60, 80 min and reperfusion for an additional 20 min resulted in a decrease in both SOD and GSH-Px activity. SOD activty decreased continuously when the hearts perfused in total ischemia for periods of 40 and 60min were reperfused for an additional 20min. GSH-Px activity was much lower during reperfusion(total ischemia 40 min and reperfusion 20 min) than that during total ischemia(60 min) and ultrastrcture damage in this phase was the most severe. Both sodium selenite(Na_2SeO_3 3?10~(-8)M)and DS-182(4?10~(-6)g/ml, one of the effective components of salvia miltiorrhiza bunge) could increase the activity in both SOD and GSH-Px and protect the ultrastreture of myocardium from reperfusion injury. The DS-182 is more effective than Na_2SeO_3 in the protection of myocardium from reperfusion damage.