Study on anoxia-reoxygenation injury and effects of copper and zinc in heart cell cultures / 中国病理生理杂志

ABSTRACT

The effect of various concentrations of copper and zinc on anoxia-reoxygenation in the neonatal rat heart cell cultures was studied by determining the activity of superoxide dismutasc (SOD), glutathione peroxidase (GSH-px), the content of malondialodchyde (MDA) and the release of lactate dehydrogenasc (LDH) The results showed that teh activity of SOD decreased and the activity of GSH-px, the content of MDA and the release of LDH increased following anoxia and reoxygenation. The actiwty or SOD appeared to be increased by either copper or zinc to varying degrees, but the effect of copper showed to be stronger than that of zinc. The release of LDH was reduced at the presence of lower amounts of copper or zinc, but the effect of zinc was better than that of copper. The increased release of LDH occured when higher amounts of copper or zinc were added to the medium. In addition, copper increased the activity of GSH-px while reducing the content of MDA significantly.