An improved ELISA for Detection of Monoclonal Antibodies to Human Hepatocellular Carcinoma / 昆明医科大学学报
Journal of Kunming Medical University
; (12)1988.
Article
in Zh
| WPRIM
| ID: wpr-515798
Responsible library:
WPRO
ABSTRACT
Instead of poly—I—lysine, the microtitre plate is in turn pretreated with concentrated sulphuric acid and 100?g/ ml protamine. The living target cells are well attached to the plate. Fixed with 0.025—0.05% glutaraldehyda, the target cells are suitable for screening monoclonal antibodies by ELISA. This assay is specific, sensitive and well reproductive. It is a useful method for screening large scale spent supernatant of hybridomas and ascites at less expense and time. On the other hand, this method can be also used to detect cellular antigens with either MAbs or conventional antibodies.
Full text:
1
Index:
WPRIM
Type of study:
Diagnostic_studies
Language:
Zh
Journal:
Journal of Kunming Medical University
Year:
1988
Type:
Article