Effects of ascorbic acid and cortisol on the antigen-presenting function (APF) of normal human monocytes / 中国病理生理杂志
Chinese Journal of Pathophysiology
; (12)1986.
Article
in Zh
| WPRIM
| ID: wpr-515891
Responsible library:
WPRO
ABSTRACT
Mononuclear cells obtained from heparinized fresh venous blood of healthy male blood donors by means of Ficoll-Hypaque densitz gradient centrifugation were subjected to petri dish adhesion and nylon-wool-column separation to harvest monocytes (Mon) and T lymphocytes (T) high purity, respectively. APF of Mon, as reflected by ~3H-TdR incorporated T proliferation, was assayed after the Mon had been preincubated with PPD (purified protein derivative, end concentration 9.25 ?g/ml), PPD+ascorbic acid, PPD+cortisol, and PPD+ascorbic acid+cortisol for 24 hours at 37℃, in a humidified incubator containing 5% CO_2. It was shown that APF of the Mon was markedly enhanced by ascorbic acid in a concentration of 3.75mM (P0.05).
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Index:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Pathophysiology
Year:
1986
Type:
Article