The cytotoxicity of scorpion venom (buthus martensii karshi) on human esophageal cancer cell——Eca109 cultured in vitro / 中国病理生理杂志

ABSTRACT

The cultured human esophageal cancer cell line——Eca109 cells was incubated with the scorpion venom crude (SVC) collected from Buthus Martensii Karshiin Henan Province. The growth inhibition and cytotoxicity of SVC on Eca109 cells were detected. The results showed that Eca109 cell growth was inhibited by SVC. while Eca 109 cells were incubated with SVC for 24, 48, 72hrs. The rates of inhibition were 35.6%, 39.5, 36.9% respectively, the concention of SVC used was 0.017?g/ml. The mitochondrial dehydrogenase of Eca109 cells were also inhibited by SVC, Which had the cytotoxic effect on Eca109 cells. When the concentrations of SVC were 0.017?g/ml, 0.034?g/ml, 0.085?g/ml, the cytotoxicity were 63%, 56% and 59%, respectively. The effect and mechanism of cytotoxicity of SVC on the tumor cells are worth further studies.