Establishment of a Human Lymphoblastoid Cell Line from Human Lymph Node / 昆明医科大学学报

ABSTRACT

A human lymphoblastoid cell line named KGHLY, which has been established from a human lymph node, is reported in this paper. KGHLY grew in suspension and formed clamps easily. It grew rapidly and the population doubling time of the 30th generation cells was 26 hours. Under the light and electron microscope the culture cells were similar to lymphoblastoid cells in morphology and structure. The cells secreted IgM. Surface marker studies showed that there were no receptors for sheep erythrocytes. The results suggested that the cells derived from B lymphocytes. The karyotype was aneuploid with a modal chromosome number of 44—46. It has lasted for over one year through seventy passages. The cells stored in liquid nitrogen and their revival was fine. This cell line will be available material for the study of lymphoblastoid cells and human-human hybridoma.