The effects of several tumor promoters on gap junction intercellular communication in NIH/3T3 cells / 中国病理生理杂志

ABSTRACT

The effects of tumor promoter TPA, MZR and PB on gap junction intercellular communication in NIH/3T3 cells were studied using the scrape-loading/dye transfer technique. All of these 3 agents were shown to cause a dose-dependent inhibition of the intercellular communication. The blockage of intercellular communication induced by TPA and MZR was inhibited in the presence of the protein kinase C inhibitor, staurosporine or palmitoyl carnitine, but not inhibited in the casexinduced by PB. The results suggest that the serape-loading/dye transfer technique may be used as a rapid screening assay to detect the tumor promoters and protein kinase C may be involved in the genesis of intercellular communication blockage induced by TPA ane MZR, but not by PB.