Effect of nonenzymatically glucosylated low density lipoprotein on in vitro cultured macrophages and monocytes / 中国病理生理杂志

ABSTRACT

We observed the catabolic characterestics of in vitro prepared nonenzymatically glucosylated low density lipoprotein (glcLDL) in mouse peritoneal macrophages (M?) and human monocyte line (U_(937); and found that glcLDL did not stimulate the increase degradation of glcLDL significantly nor did the accumulation of glcLDL cholesterol in mouse peritoneal M?. However the degradation of glcLDL by U_(937) cells was positively proportional to the concentration of glcLDL. The pre-treatment of U_(937) cells with 12-O-tetradecanoylphorbol 13-acetate (TPA) increased the degradation of glcLDL and was also positively proportional to the concentration of glcLDL. The relationship between these phenomena and the accelerated developement of atherosclerosis in diabetes mellitus was discussed.