Establishment and identification of L783V/3T3 cell line infected with leukemia viruses / 中国病理生理杂志

ABSTRACT

L783 murine lymphocytic leukemia was established in 1978 by Dept. of Pathophysiology of Shanghai Medical University. L783V/3T3 cell line was established by infecting NIH-3T3 cells with the cell-free extracts of blood, spleen respectively of L783 mice. The plaque assay of L783V/3T3 cells was positive, typical A-type and C-type virus particles could be observed under electronmicroscope. Lymphocytic leukemia could be induced by inoculation of cell-free extracts from L783V/3T3 cells into Kunming strain newborn mice.