Determination of Emodin and Chrysophanol in Shenqu Weitong Capsules by RP-HPLC / 中国药房

ABSTRACT

OBJECTIVE: To determine the contents of Emodin and Chrysophanol in Shenqu weitong capsule by RP-HPLC. METHODS: The samples were separated on Phenomenex BDS-C18(250 mm?4.6 mm, 5 ?m).The mobile phase con-sisted of acetonitrile-water-glacial acetic acid (55∶45∶0.5) at a flow rate of 1.0 mL?min-1. The detection wavelength was set at 254 nm. RESULTS: The linear range for Emodin was 2.188～54.700 ?g?mL-1(r=0.999 9) and 4.312～107.800 ?g?mL-1 for Chrysophanol (r=0.999 9). The average recovery of Emodin was 96.52% (RSD=0.59%) versus 98.45%(RSD=0.43%) of Chrysophanol. CONCLUSION: This method is convenient, accurate, reproducible and applicable for the quality control of Shenqu weitong capsule.