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Preparation of Desmoglein 1,3 Type Probes Using PCR of Incorporation of Dig-dUTP Method / 中华皮肤科杂志
Chinese Journal of Dermatology ; (12)1995.
Article in Chinese | WPRIM | ID: wpr-516591
ABSTRACT
In this study we successfully prepared desmoglein 1 and desmoglein 3 DNA probes by incorporation of Dig-dUTP during PCR amplification. Eleetrophoresis analysis showed that Dig labelled probes moved slower on the gel than PCR products not incorporated with Dig due to the enlargement of probe fragment. The results of Dot-blot showed that the prepared probes can be used for RNA analysis, and both probes have high specificity, The results provided basis for the quantitative and qualitative study of desmoglein gene expression in tissues or cells by means of in situ hybridization and Northern hybridization etc.

Full text: Available Index: WPRIM (Western Pacific) Type of study: Qualitative research Language: Chinese Journal: Chinese Journal of Dermatology Year: 1995 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Type of study: Qualitative research Language: Chinese Journal: Chinese Journal of Dermatology Year: 1995 Type: Article