Effects of clonidine on releases of enkephalin and substance P from periaqueductal gray / 中华麻醉学杂志

ABSTRACT

Objective:The stimulative effects of clonidine on release of enkephalin and substance P from periaqueductal gray was studied to analyse the analgesic neurotransmitters related to alpha 2 adrenoceptor activation. Method: Twenty SD rats were allocated in pairs to receiving intraperitoneal clonidine 40?g in clonidine group or normal saline in control group. Immunohistochemical technique was used to measure the contents of enkephalin and substance P in slice of rat midbrain. Result:The immunoreaction of enkephalin from slice of rat midbrain was decreased significantly in clonidine group compared with control group(P