The effect of human complement C_(5b~9) complex on nitric oxide synthesis in glomerular mesangial cells of rats / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)1986.
Article
in Chinese
| WPRIM
| ID: wpr-516924
ABSTRACT
AIM:
To study the effect of human complement C 5b~9 complex on nitric oxide(NO) synthesis of glomerular mesangial cells (MC).METHODS:
First, the human complement C 5b~9 complexes were isolated and glomerular MC of rats were cultured. Second, the MC were stimulated with C 5b~9 complex and changes of metabolism products of NO(NO 3 and NO 2) in MC culture supernatant at 6,24 and 48 hours after C 5b~9 stimulating were detected. Moreover, cGMP levels in cultured MC were also measured.RESULTS:
NO 3/NO 2 contents from culture supernatant and cGMP levels in MC were increased parallelly after C 5b~9 complex stimulation. Further, NO synthesis was inhibited by L-NG-nitro-arginine-methylester(L-NAME).CONCLUSION:
NO synthesis of rat glomerular MC was incerased by human complement C 5b~9 stimulation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Pathophysiology
Year:
1986
Type:
Article
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