Effect of NO signal system on rat airway ciliary motility / 中华麻醉学杂志

ABSTRACT

Objective To understand the effects of NO signal system on the ciliary beating frequency (CBF) of airway epithelial cellMethods Nine normal male Sprague-Dawley rats were anesthetized with isoflurane Their tracheas were rapidly removed using aseptic technique The mucosa of trachea were cut into 1mm2 explants and cultured in DMEM The explants were divided into 5 groups as bellow L-Arg group, 1-Hydroxy-2-oxo-3-(N-ethyl-2-aminoethyl)-3-ethyl-1-triazene (NOC-12) group, D-Arg group, 8-Br-cGMP group, and phosphate buffered saline (PBS)group Actively beating ciliated cells were observed, and their motion was quantified by measuring CBF using phase-contrast microscopy and videotape analysis Results L-Arg increased CBF from (7 43?0 75)Hz to(8 59?0 93)Hz (P