Immunohistochemical study on ACTH cells and TSH cells of pituitary pars distails of morphine dependent male rats and its restoration after withdrawal / 中国病理生理杂志

ABSTRACT

AIM: To investigate the change of adrenocorticotropic hormone (ACTH) cells and thyroid- stimulating hormone(wn) cells of pars distalls of pituitary gland of morphine dependent dependent rats and its restoration after withdrawal. METHODS: Morphine dependent model of male rats was made by subcutaneous injection of mor- phine and the adstinent model was made after withdrawal. Changes of ACTH cells and TSH cells of pars distails of pituitary gland were detected by immunohistochemistoy survey and image analysis. RESULTS: The intensity of positive staining and the numerical density of ACTH and TSH immunoreactive cells were weakened (P＜ 0.01), after withdrawal from morphine for a short time the changes would exist continuously. CONCLUSION: Morphine may give rise to disorder of endocrine of pituitary gland of male rats, which may incompletely restore after withdrawal for a short time.