L-dopa induction of pheochromocytoma12 cells apoptosis and the protective function of glutathione / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)1989.
Article
in Chinese
| WPRIM
| ID: wpr-516993
ABSTRACT
AIM:
To study the induction of apoptosis of pheochromocytoma (PC12) cell by L - dopa and the clinical significance.METHODS:
Using PC12 cells as the medel of dopaminergic neurons,in addition to electron microscopy, agarose gel electrophoresis and flow cytometry, the apoptosis ratio by L - dopa and the effect of antioxi- dant glutathione on PC12 cells were observed.RESULTS:
Treabent of PC12 cells with L - dopa in concentrations of 50 ?mol/L, 100?mol/L and 150 ?mol/L respectively for 24 hours, results revealed that the apoptosis ratio was 12. 8%, 24.4%, 37. 2%, respectively, which is in cobant with fragment pragment propartions of agarose gel electrophoresis, mere higher than that of control(2. 3% ) (P 0 .05). CONCLUTION Induction of apoptosis by L - dopa could be inhibited by glutathione, and oxidative damage may be involved in the pathophysiology of dopaminergic neurons death after long - term treat- ment of L - dopa.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Pathophysiology
Year:
1989
Type:
Article
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