Myocardial protection by desflurane from anoxia/reoxygenation injury via activation of K_(ATP) channels / 中华麻醉学杂志

ABSTRACT

Objective To evaluate the roles of K ATP channels in desflurane-induced myocardial protection from anoxia/reoxygenation injury Methods Primary cultured rat myocardial cells were randomly allocated to four groups control group(A) without any treatment; anoxia/reoxygenation group(B) reoxygenation of 1 h following anoxia of 2 h; desflurane preconditioning group(C) 20 min of 9% desflurane preconditioning followed by 10 min washout before anoxia/reoxygenation and K ATP channel blocker group(D) adding glybenclamide at final concentration of 12?g/ml to culture medium 10 min before the same procedures as group C The activities of lactic dehydrogenase (LDH) and creatine kinase (CK), rates of cell viability and apoptosis, contents of cellular malondiadehyde (MDA) and adenosine triphosphate (ATP), and intracellular free calcium concentration were measured Results Compared with control group, anoxia/reoxygenation caused great increases of levels of LDH, CK, apoptosis and MDA ,and decreases of ATP and cell viability (P