Intracellular calcium alterations induced by endotoxin and IL-1? in rabbit hypothalamic neurons / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12)2000.
Article
in Chinese
| WPRIM
| ID: wpr-517074
ABSTRACT
AIM:
To investigate intracellular free calcium ([Ca 2+ ]i ) alterations in hypothalamus of febrile rabbits induced by endotoxin (ET), and compare with the effect of ET and IL-1? on [Ca 2+ ]i in hypothalamic neurocytes from normothermia rabbits.METHOD:
The concentration of [Ca 2+ ]i was determined by using spectrofluorometer and fluorescent Ca 2+ probe fura-2 /Am.RESULTS:
1. A minute dose of ET (2 ng/mL) induced a significant rise in [Ca 2+ ]i in hypothalamic neurocytes from normothermia rabbits. The rise in [Ca 2+ ]i in hypothalamic neurocytes from febrile rabbits induced by intravenous injection of ET was also observed. 2. In hypothalamic neurocytes from normotheria rabbits, IL-1? failed to affect [Ca 2+ ]i at concentrations of 100, 500, 1 000 ng/mL, respectively.CONCLUSION:
The action site of low concentration of calcium that plays a regulatory role during fever seems unlikely to be in cytosolic compartment of hypothalamic neurons. The change of [Ca 2+ ]i in hypothalamic neurocytes by ET can not be considered the direct effect of IL-1?.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Pathophysiology
Year:
2000
Type:
Article
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